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For elution under hybrid conditions, the column was washed three times with 200 mL of native wash buffer (50 mM NaH₂PO₄, 300 mM NaCl, 20 mM imidazole, pH 8.0), removing residual urea

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Conclusion: It is difficult to use screening to predict which children and adolescents will have high blood pressure as adults, there is limited information on blood pressure cuff accuracy in the diagnosis of hypertension in children and adolescents, and evidence of inappropriate use, incorrect calibration, or lack of this material in outpatient clinics