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glutamine is a particularly critical nutrient sensed in late G1 phase, and its deprivation causes cell cycle arrest at G1 phase 186

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In all experimental systems, including 2D cell lines, spheroids, organoids, xenografts, residual disease models, and clinical samples, DTP cells consistently showed survival under high drug concentrations or prolonged exposure, depending on non-genetic adaptive programs, and recovery of proliferative potential and drug sensitivity after treatment cessation