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* Correspondence: Ruedeemars Yubolphan, [email protected] Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers

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Key Takeaways AHK (Tripeptide-3) standard topical dosing ranges from 2-10 mg per application, typically applied 1-2 times daily The tripeptide has a molecular weight of 340.38 Da and demonstrates enhanced skin penetration when formulated at concentrations between 0.1-1% [1] Research protocols typically utilize 28-56 day application cycles with 2-week rest periods between cycles Bioavailability through topical administration is approximately 15-25% compared to subcutaneous injection routes [2] AHK (Tripeptide-3) is classified as research-only and not FDA-approved for therapeutic use Clinical supervision is essential due to limited human safety data and variable individual response patterns Half-life studies indicate tissue retention of 4-6 hours following topical application, influencing twice-daily dosing recommendations [3] How AHK (Tripeptide-3) Works in the Body AHK (Tripeptide-3) functions through activation of transforming growth factor-beta (TGF-β) signaling pathways and upregulation of antioxidant enzyme systems