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Generation of the standard calibration curve From the secondary standard solution, aliquots of 0.25, 0.5, 0.6, 0.75, 1, 1.25, 1.5, 1.75, 2, 2.25, and 2.5 mL were pipetted and transferred to a series of pre-labelled test tubes and made up to the 5 mL mark with methanol to obtain a 5, 10, 12, 15, 20, 25, 30, 35, 40, 45, and 50 µg/mL calibration standard

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The P300/XBP1s/Herpud1 axis promotes macrophage M2 polarization and the development of choroidal neovascularization