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Preparation of Whole Cell Lysates and Nuclear Extracts Whole cell lysates were prepared in RIPA buffer containing 50 mM Tris-HCl pH=8.0, 150 mM NaCl, 1% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS supplemented with freshly added protease and phosphatase inhibitors (Merck, Darmstadt, Germany)
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Red meat, poultry, and fish are the best dietary sources of l-carnitine
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