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For protein heat inactivation, tissue homogenates were incubated at 95 °C for 1 h

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FIGURE 2 3.3 Evaluation of GSH chromatography residence time and binding capacity GSH affinity chromatography parameters (Supplementary Figure S1) were investigated using both 37°C and 34°C infection materials to maximize infectivity yield and to determine operational conditions that may improve empty capsid clearance in 34°C infection harvests