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Cells were resuspended in Ran Lysis Buffer (50 mM KPO 4, pH 7.0, 250 mM NaCl, 2 mM MgCl 2 , 1 mM PMSF, and 3 mM 2-mercaptoethanol) and, after sonication, the soluble fraction was bound to low-density nickel agarose beads (GoldBio)
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At last, the binding conformations of the top 2,000 compounds from Glide XP were exported, including the binding conformation of compound 102
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