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Finally, AsA / V E was calculated for each group ( 2.9 Glutathione content measurement Tissue samples (1.5 g) were ground in 2 mL 60 mmol L −1 sodium phosphate buffer (pH 7.0), and refrigerated centrifugation was performed at 12,000 × g for 15 min, after which 100 μL of supernatant was obtained