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Table (3): ADME validation of the metabolites from Burkholderia stagnalis Selected bioactive metabolites obeyed ‘Lipinski’s rule of 5’ without any violation suggesting their possibility of oral administration, The boiled egg plot evaluate the pharmacokinetic properties of the 24 bioactive metabolites, helping to predict their bioavailability and potential therapeutic applications (Figure 6)

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In contrast, pathogen-associated molecular patterns (PAMPs) are conserved molecular structures found in certain microbes for the innate immunity, leading to responses such as cytokine and interferon production, helping to protect the host from infection