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In contrast, one study showed that the intake of unfiltered Turkish coffee not only had no protective effect on CCl 4 -induced liver fibrosis but also significantly increased AST/ALT levels, thereby exacerbating CCl 4 -induced hepatotoxicity ( Persistent inflammatory factors stimulate HSCs in the liver to secrete fibrotic factors that increase extracellular matrix (ECM) formation and ultimately lead to liver fibrosis ( 4 , DMN, and TAA-induced hepatic fibrosis, coffee and caffeine intake reduced transforming growth factor-β (TGF-β) levels and thus inhibited HSC activation and proliferation ( in vivo studies have shown that the anti-fibrosis properties of caffeine converge with a reduction in HSC activation and proliferation ( HSCs are deemed the main effector cells in liver fibrosis (Yang et al., 2015)

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Individuals with chronic liver conditions sometimes use these injections to support liver detox, though scientific evidence remains limited as referenced by the American Academy of Dermatology and the National Institutes of Health