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Because peptides are susceptible to enzymatic degradation by proteases present in serum, researchers often perform migration assays in low-serum (e.g., 0.5% to 1% fetal bovine serum) or serum-free conditions

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FOXO4-p53 competition → displacement of p53 from nuclear sequestration Mitochondrial p53 relocation → apoptosis in senescent cells Senescence-restricted complex → viable cells largely unaffected D-retro-inverso design → side-chain topology preserved, proteases evaded Core Areas of Scientific Investigation Senolytic Research: FOXO4-DRI is a reference compound for selectively removing senescent cells in culture and rodent models