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Over the past decade, a variety of protocols have been established for the differentiation of human iPSCs into hepatocytes in vitro, either through viral-based expression of major hepatic transcription factors (3,4), using growth factors (5-7), small molecules (8,9), or different combinations of growth factors and small molecules (10,11) to recapitulate major signaling pathways involved in the different stages of embryonic hepatogenesis

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Elution was performed twice, first with 3 ml of 2 mM Tris (pH 8.0) containing 30 mM NaCl, then once again with 2 ml of the same buffer