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Numerous techniques have been developed for manufacturing liposomes, ranging from traditional methods, such as the thin-film hydration method, the detergent removal method, the solvent injection method, and the reverse-phase evaporation method, to more novel approaches, including lyophilization, supercritical fluid-assisted methods, microfluidic techniques, and membrane contractor methods [120]

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The ß-arrestins show high affinity for receptors that are both phosphorylated and activated, but are still able to bind non-phosphorylated receptors with a lower affinity