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These excess nutrients are what cause many problems, including the buildup of a sludge layer, a drop in oxygen levels, smelly odors and the rapid accumulation of floating green organic matter

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Samples were run in the pre-electrophoresis buffer for 3 h at 40 mA at 4°C and then were run in the electrophoresis buffer, pH 8.3 containing 50 mM Tris, 300 mM glycine, and 1.8 mM disodium EDTA for 3 h at 40 mA at 4°C