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The plate reader should be set to incubate the microplate at a constant temperature of 28°C during the read
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ARLD patients exhibit alterations in the morphology of hepatic mitochondria, with the pioneering description of the presence of megamitochondria (large and elongated mitochondria) as a result of an elevated mitochondrial fusion activity, which triggers mitochondrial elongation and is related to the induction of OXPHOS activity and mtDNA relocation in the mild stage of the ARLD spectrum [171]
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