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Studies have shown that, compared to the IBS-C group, the intestinal microbial composition following MJGT administration has undergone significant changes, including an increase in the Bacteroides/Firmicutes ratio and a decrease in the abundance of the Lachnospiraceae family.47 The flavonoids in Amomum tsaoko, such as (+)-epicatechin, (-)-catechin, L-epicatechin, and isoquercitrin, not only increase the 5-HT content and the expression of HTR2A, phospholipase A2, and COX-2, all of which are involved in the serotonergic synaptic pathway, but also enhance the expression of transient receptor potential A1, promoting the release of myosin light chain 3, which facilitates smooth muscle motility